Alanine aminotransferase kuchuluka - chizindikiro cha njira yotupa ndi osachiritsika ziwalo

Chifukwa aminotransferase kusamuka kwa NH2-magulu ndi asidi mwiniwake γ-kwa amino kwa acceptor - asidi α-kwa amino popanda mapangidwe wapakatikati wa ammonium. Ndiko kuti, transamination (transamination: kusintha kulanda magulu kwa amino kuti keto acid) - njira anatsegula asayansi otchuka Russian ndi Kritsman Braunstein.

amino zidulo (aspartic, glutamic ndi alanine) pa transamination n'kukhala lolingana α-keto asidi, amene zigawo za asidi mkombero tricarboxylic. Pa makutidwe ndi okosijeni awo Krebs adzizungulira, iwo ndi gwero la mphamvu.

Transamination n'kofunika kupereka wa urea mkombero aspartate.

Ngakhale kuti zochita transamination akhoza poyera ndi onunkhira kwa amino zidulo: phenylalanine, tyrosine ndi tryptophan - angagwiritsire ntchito mankhwala wapeza tanthauzo la ntchito ya aspartate ndi alanine aminotransferase. michere awa inafalitsidwa mu zimakhala m'thupi la munthu.

Alanine aminotransferase adakulabe mu maselo ofiira ndi ndende zake ndi kasanu ndi kamodzi apamwamba poyerekeza kuti ndi seramu magazi. Ndende apamwamba enzyme ndi chili mu zimakhala chiwindi.

Alanine aminotransferase ponseponse sali yemweyo kuti zimakhala thupi. Mwachitsanzo enzyme ntchito minofu mtima ndi 7.1 (E * 10-3 ga minofu homogenate), ndi chiwindi - 44, chigoba minofu - 4,8, impso - 19, ndulu - 1.2, 0,7, m'mapapo seramu - 0,016. njira zilipo kwa mtima wa ntchito aminotransferase ku seramu ali m'magulu awiri; endpoint (colorimetric, ntchito ochiritsira, osati okonzeka ndi kutentha ankalamulira cuvette photometric zida) ndi kayendedwe (spectrophotometric).

B6 ndi vitamini wamba mu zakudya zosiyanasiyana. Komabe, tiyenera kukumbukira kuti pa ndondomeko kuphika (matenthedwe obrobka) mavitamini waonongeka. Pyridoxine ndi cofactor mu michere kwambiri. Ndende pirodoksin-5-mankwala mu magazi amachepetsa ndi zaka. Choncho, ntchito ya enzyme izi alanine aminotransferase, utachepa anthu achikulire kuposa zaka 64 (pafupifupi 30%) kuposa anthu okalamba zaka 46-63. The ndende ya vitamini B6 mu magazi seramu alanine aminotransferase zimayambitsa.

Kudziwa boma la chiwindi zinchito kawirikawiri anayesedwa milingo aminotransferase mu magazi a wodwalayo. Ichi ndi chifukwa makamaka chakuti asintha ntchito yawo amapezeka mofulumira kwambiri kuposa magawo ena zasayansi. Kucotsa (kuchoka) wa michere chiwindi mu mwazi kumasonyeza kuti cytolysis - maselo kapena kusokoneza permeability awo Kakhungu. Pamene kupenda kusintha ntchito ya michere yofunika kukhazikitsa malo kuwonongedwa. Muyenera kudziwa ntchito yawo maselo a mwazi ndi thupi lathanzi, ndi kutanthauzira subcellular ndipo thupi. The puloteni ndende - alanine kuchuluka, makamaka maselo chiwindi Choncho, ngakhale pang'ono kuwononga amakwiya awo ponso ntchito enzyme m'magazi. Popeza ntchito enzyme ndi matenda matenda a chiwindi (a chiwindi, steatosis).

Alanine aminotransferase kuchuluka necrosis a chiwindi, komanso parenchymal chiwindi. The otsika ndende ya enzyme ndi mayina a chiwindi aakulu dystrophy. Izo ziyenera kuti akuchuluka mu ntchito aminotransferase ku seramu akuyamba masiku 3-8 pamaso pa isanayambike waukulu zizindikiro matenda a matenda ndi kufika pazipita masiku oyamba a chitukuko cha ndondomeko pathological.

Choncho, alanine aminotransferase kuchuluka: matenda a chiwindi, matenda a chikasu ndiyo, khansa chiwindi, m'mnyewa wamtima infarction, pakali mtima kulephera, hypoxia, mantha, myositis, myocarditis, steatosis, pancreatin, aakulu uchidakwa.