Zonse zokhudza puloteni ya pepsin

M'nkhani ino tidzakambirana za mthendayi yodalirika, yomwe ili m'mimba mwa nyama iliyonse, kuphatikizapo munthu. Zambiri zokhudza puloteni ya pepsin, chidziŵitso cha isomers ndi gawo la mankhwala mu chimbudzi njira zidzalingaliridwa.

Maganizidwe ambiri

Choyamba, tiyeni tipeze mtundu wa mapuloteni a pepsin. Izi zimapereka kumvetsetsa kwakukulu kwa mutu womwewo.

The enzyme pepsin imatchula proteolysis class hydrolases ndipo imapangidwa ndi chapamimba mucosa, ndipo ntchito yake yaikulu ndi chiwopsezo cha mapuloteni omwe amaperekedwa zakudya kwa peptides. Pepsin ndi puloteni yomwe imathyola mapuloteni m'mlengalenga. Zimapangidwa ndi zamoyo zonse zakutchire, komanso zinyama, oimira gulu la mbalame ndi nsomba zambiri.

Puloteni yamakonoyi ndi mapuloteni a mtundu wa globular, ali ndi maselo pafupifupi 34,500. Molekyu wokha imayimilidwa ngati unyolo wa polypeptide ndipo umakhala ndi mazana atatu makumi anai amino acid. Zomwe zimapangidwanso zili ndi HPO3 ndi zitatu disulfide bonds.

Pepsin amagwiritsidwa ntchito kwambiri pakupanga mankhwala ndi tchizi. Mu ma laboratories amagwiritsidwa ntchito pofuna kufufuza mwatsatanetsatane mankhwala a mapuloteni, omwe ndi, mapuloteni oyambirira. Pepsin ali ndi chilema chachilengedwe - pepstatin.

Mapuloteni osiyanasiyana

Pepsin ali ndi isoforms khumi ndi ziwiri. Kusiyanitsa pakati pa isomers onse a pepsin ali mu mphamvu ya electrophoretic motor, machitidwe osokoneza, ntchito zowonongeka. Mphuno ya pepsin ndi CF 3. 4. 23. 1.

Munthu m'mimba mwa m'mimba ali ndi mitundu isanu ndi iwiri ya pepsin, ndipo asanu mwa iwo amasiyana kwambiri ndi makhalidwe ena:

1. Pepsin palokha (A) imakhala ndi ntchito yaikulu pa pH = 1.9, ndipo ikaukitsidwa mpaka 6 imachotsedwa.
2. Pepsin 2 (B) imapangidwira pH = 2.1.
3. Mtundu 3 umasonyeza ntchito yaikulu pa pH = 2.4-2.8.
4. Mtundu 5, wotchedwanso gastricin, uli ndi ntchito yaikulu pa pH = 2.8-3.4.
5. Pangani 7 ndi pH chikhalidwe cha 3.3-3.9 chiri ndi ntchito yaikulu.

Mtengo wa enzyme mu chimbudzi

Pepsin amavomerezedwa ndi ziwalo za m'mimba mwa njira yosaimitsidwa (pepsinogen), ndipo hydrochloric acid imayambitsa mavitamini omwewo. Pogwiritsa ntchito mphamvu yake, imadutsa mu mawonekedwe odabwitsa. Chinthu chofunika kwambiri pa ntchito ya pezyin ya enzyme ndi kukhalapo kwa sing'anga, chifukwa chake, patsiku la pepsin kulowa mu duodenum, imataya ntchito yake, popeza m'matumbo sing'anga ndi amchere. The enzyme pepsin ndi imodzi mwa maudindo ofunika kwambiri mu chimbudzi cha gulu lonse la zinyama, makamaka anthu. Izi zimapangitsa mapuloteni a zakudya kuti azikhala ndi zingwe za peptide ndi amino acid.

Mwa amuna ndi akazi, kupanga kwa enzyme imeneyi kumasiyana. Amuna ali ndi magalamu makumi awiri mpaka makumi atatu a pepsin pa ora, pamene mkazi ali ndi makumi awiri mpaka makumi atatu peresenti yochepa. Maselo akulu, malo opangira pepsin, amawagawa mu mawonekedwe osavuta a pepsinogen. Pambuyo pa chiwindi cha peptides kuchokera kumapeto kwa N-terminal, pepsinogen imakhala yogwira ntchito. Hydrochloric acid imakhala ngati chothandizira pa zomwe zimachitika kusintha kwa mankhwala. Pepsin ili ndi protease ndi peptidase ndipo imayambitsa kusagwirizana kwa mapuloteni.

Thandizo

Mu mankhwala, pepsin amagwiritsidwa ntchito kwambiri ngati mankhwala a matenda enaake omwe akugwirizana ndi kusowa kwa mankhwalawa mu mimba ya wodwala. Pezani tsamba la rennet pepsin kuchokera ku porcine mucous membranes m'mimba. Mankhwalawa amapezeka ngati mapiritsi, amafalikira pamatenda, ndi kusakaniza kwa acidol kapena mawonekedwe a ufa. Pepsin ndi mbali ya mankhwala ena. Ali ndi ATS code A09AA03. Chitsanzo cha matenda omwe mankhwala a pepsin amalembedwa ndi Menetrie's disease.

Nkhumba ya pepsin ndi ...

Rennet pepsin rennet ndi imodzi mwa mitundu yomwe imadziwika ndi yofala kwambiri. Enzyme yomwe imadzipangidwira m'mimba ya ng'ombe yachinayi. Kukonzekera komwe kumagwiritsidwa ntchito pakupanga kumapangidwira ndi michere iwiri: pepsin ndi chymosin mumtundu wofanana. Ntchito ya enzyme yogwiritsiridwa ntchito rennet mu kupanga tchizi, ndi ntchito zake zazikulu - kupanga kapangidwe ka mkaka komanso kutenga nawo mbali mukusakaniza kwa tchizi ndi zowonongeka.

Nkhumba za pepsin zimachokera m'mimba mwa ng'ombe komanso popanga mankhwala ogulitsidwa, pali magawo awiri oyeretsa mavitamini omwe ali ndi mafuta ndi zosafunika zomwe sizingatheke. Ntchito yopanga njuchi ya pepsin imadutsamo miyeso yambiri: kuchotsa mchere, kuyiritsa mchere komanso kuyanika.

Ntchito zina

Enzyme pepsin imaphatikizidwira kuyambira. Amagwiritsidwanso ntchito popanga tchizi. Enzyme ya rennetin yokhala ndi chymosin imapangitsa kuti puloteniyo ipange mkaka.

Mchitidwe wophimba mkaka umatchedwa mapuloteni coagulation, omwe ndi casein, ndi kupanga gelisi pa mkaka. Casein ali ndi chingwe chokhazikika, ndipo pepala imodzi yokha imagwirizanitsa ndi mapuloteni okhawo. Pepsin yokhala ndi chymosin yoyenera komanso yothandizira kuwonongeka kwa mgwirizano womwe umayambitsa kupaka mkaka.

Kutsiliza

Kufotokozera mwachidule, zimatchulidwa kuti izi ndizofunikira kwambiri m'magulu opanga zakudya m'mimba mwa oimira magulu ambiri a zamoyo. Pogwiritsa ntchito mankhwala ndi mankhwala, mankhwalawa amagwiritsidwa ntchito ngati mankhwala ndipo amawonjezeredwa ku enzyme ya rennet kuti apange mkaka.